Tissue Specific Esterase Isozyme Banding Pattern in Nile Tilapia (Oreochromis niloticus)
نویسندگان
چکیده
The electrophoretic banding pattern of esterase isozymes were examined in eighteen different tissues of Nile tilapia after staining with α and β naphthyl acetate as substrate. The tissues were anterior-, mid-, tailmuscle (ventral and tip region), buccal muscle, stomach, fore-, mid-, hind-gut, liver, gill, heart, kidney, eye (lens), eye (iris), fore-, midand hindbrain. Altogether five bands named as Est-1, Est-2, Est-3, Est-4 and Est-5 were observed different relative mobility. Est-1 and Est5 denote the fastest and slowest band. Est-3 was present in all the tissues. All five bands were expressed in liver and stomach. Some of the esterase bands showed tissue and substrate specificity, where Est-1 was in fore-, midand hindbrain, Est-2 in hind gut, Est-5 in stomach, gill and heart was stained only with α naphthyl acetate. None of the band was expressed with β naphthyl acetate only.
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